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Discussion in 'All Categories' started by tameika - Oct 5th, 2012 5:59 am.
i did a herpes test the range was 1.1. one of the doctor said i need to take over the test cause the range is low and the other clearly states its positive. i would like to know the cut off range and if this could be a false positive.
re: heprpes by Dr J S Chowhan - Oct 13th, 2012 3:01 pm
Dr J S Chowhan
Dr J S Chowhan
Dear Tameika

A positive herpes simplex culture or HSV DNA test from a vesicle scraping indicates an active HSV-1 or HSV-2 infection. A negative test result indicates that the herpes simplex virus was not isolated but does not definitely rule out the presence of virus. This is because if the specimen taken does not contain actively replicating virus or if the sample was not transported under optimum conditions, no viable virus may be detectable, resulting in a false negative result.

The presence of HSV-1 or HSV-2 IgM antibodies indicates an active or recent infection. HSV-1 or HSV-2 IgG antibodies indicate a previous infection. A significant increase in HSV IgG antibodies, measured by comparing acute and convalescent samples, indicates an active or recent infection. Negative HSV antibody results mean that it is unlikely that the person has been exposed to HSV or that the body has not had time to begin producing HSV antibodies.

It depends on how the test is made. EIA tests work by placing a sample of diluted plasma in a well with a plastic bead that is covered with the antigen found on the virus the test is looking for. If the person providing the sample is positive, the antibodies in their blood will bind to the antigen on the bead. If there are no antibodies present, nothing will happen.

Here's where it gets a little complicated. Most EIA tests are non-competitive. The sample is incubated with the bead for a while, then the bead is washed. Then a conjugate is added, which will bind to any antibodies that have bound to the bead. Again, if no antibodies were present, nothing happens. The bead is washed again, and a substrate is added. If there is a conjugate-antibody-antigen sandwich present, the whole thing turns yellow. The more of that sandwich that is there, the darker the color will be. When it is read by a spectrophotometer, the intensity of the color is given a numerical value. The higher the number, the more positive the result.

With regards
JS chowhan
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